Education

PhD, ComUE Normandie Université (France), 2020

MSc, University of Caen Normandie (France), 2016

BSc, University of Caen Normandie (France), 2014

Research Interest

My research focuses on virology, molecular biology, and infectious disease diagnostics in both animal and human health. I study viral pathogenesis, host-pathogen interactions, the influence of host factors, antiviral drug screening, and the development of innovative diagnostic tools for infectious diseases. My work has involved investigating various pathogens, including equine herpesviruses, equine infectious anemia virus, equine arteritis virus, and SARS-CoV-2, using advanced molecular and cellular techniques. Additionally, I am using both bulk RNA sequencing and single-cell RNA sequencing to analyze gene expression and cellular responses in the context of viral infections.

Awards and Honors

2023, Best Poster Presentation Award, Society of Phi Zeta, LSU School of Veterinary Medicine

2018, Best Poster Award Certification 4th Conference on Impedance-Based Cellular Assays Edinburg, Scotland

2018, Second place in the French national competition: My thesis in 180 seconds (horse industry section), Paris, France

Selected Publications

Thieulent C. J., Balasuriya U. B. R., Tseng A., Crossland N. A, Stephens J. M., Dittmar W., Staszkiewicz J., Richt J. A. and Carossino M., 2025. Diabetes Exacerbates SARS-CoV-2 Replication Through Ineffective Pulmonary Interferon Responses, Delayed Cell-mediated Immunity, and Disruption of Leptin Signaling. Front. Cell. Infect. Microbiol. 15:1513687. http://doi:10.3389/fcimb.2025.1513687 (in press)

Saade D. I., Chin-Chi L., Mills E. P., Stanfield B., Thieulent C. J., Chouljenko V. N., Emelogu U., Carter R. T., Camacho-Luna P., Lewin A. C. Replication kinetics and cytopathic effect of feline calicivirus in feline corneal epithelial cells. Veterinary Ophthalmology. 2024;00:1–11. http://doi.org/10.1111/vop.13289 

Normand C., Thieulent C. J., Fortier C., Sutton G., Senamaud-Beaufort C., Jourdren L., Blugeon C., Vidalain P.-O., Pronost S.*, Hue E. A screening study identified decitabine as an inhibitor of Equid Herpesvirus 4 that enhances the innate antiviral response. Viruses 2024, 16, 746. https://doi.org/10.3390/v16050746 

Hassanien R. T., Thieulent C. J., Carossino M., Li G., Balasuriya U. B. R. Modulation of Equid Herpesvirus-1 Replication Dynamics In Vitro Using CRISPR/Cas9-Assisted Genome Editing. Viruses, 16, 409. https://doi.org/10.3390/v16030409 

Thieulent C. J., Carossino M., Peak L., Wolfson W., Balasuriya U. B. R. Development and validation of multiplex qPCR/RT-qPCR assays for simultaneous detection of SARS-CoV-2 and pathogens associated with feline respiratory disease complex. PLoS ONE. https://doi.org/10.1371/journal.pone.0297796 

Thieulent C. J., Carossino M., Peak L., Wolfson W., Li G., Balasuriya U. B. R. Complete genome sequences of two strains of canine pneumovirus derived from dogs with upper respiratory disease in the United States. Microbiology Resource Announcements (MRA). https://doi.org/10.1128/mra.01057-23 

Thieulent C. J., Carossino M., Peak L., Strother K., Wolfson W., Balasuriya, U.B.R., 2023. Development and Validation of a Panel of One-Step Four-Plex qPCR/RT-qPCR Assays for Simultaneous Detection of SARS-CoV-2 and Other Pathogens Associated with Canine Infectious Respiratory Disease Complex. Viruses 15, 1881. https://doi.org/10.3390/v15091881 

Thieulent C. J., Carossino M., Peak L., Wolfson W., Balasuriya U.B.R., 2023. Multiplex One-Step RT-qPCR Assays for Simultaneous Detection of SARS-CoV-2 and Other Enteric Viruses of Dogs and Cats. Viruses 15, 1890. https://doi.org/10.3390/v15091890 

Carossino M., Balasuriya U. B. R., Thieulent C. J., Barrandeguy M. E., Vissani M. A., Parreno V., 2023. Quadruplex Real-Time TaqMan® RT-qPCR Assay for Differentiation of Equine Group A and B Rotaviruses and Identification of Group A G3 and G14 Genotypes. Viruses 15, 1626. https://doi.org/10.3390/v15081626 

Thieulent C. J., Dittmar W., Balasuriya U. B. R., Crossland N. A., Wen X., Richt J. A., Carossino M., 2023. Mouse-Adapted SARS-CoV-2 MA10 Strain Displays Differential Pulmonary Tropism and Accelerated Viral Replication, Neurodissemination, and Pulmonary Host Responses in K18-hACE2 Mice. mSphere 2;e0055822. https://doi/10.1128/msphere.00558-22 

Thieulent C. J., Carossino M., Balasuriya U., Graves K., Bailey E., Eberth., J., Canisso I., Andrews F., Keowen M., Go Y.-Y., 2022. Development of a TaqMan® Allelic Discrimination qPCR Assay for Rapid Detection of Equine CXCL16 Allelic Variants Associated With the Establishment of Long-Term Equine Arteritis Virus Carrier State in Stallions. Frontiers in genetics 13;13:871875. https://doi.org/10.3389/fgene.2022.871875 

Thieulent C. J., Sutton G., Toquet M.-P., Fremaux S., Hue E., Fortier C., Pleau A., Deslis A., Abrioux S., Guitton E., Pronost S., Paillot R., 2022. Oral Administration of Valganciclovir Reduces Clinical Signs, Virus Shedding and Cell-Associated Viremia in Ponies Experimentally Infected with the Equid Herpesvirus-1 C 2254 Variant. Pathogens 4;11(5):539. http://10.3390/pathogens11050539 

Paillot, R., Sutton, G., Thieulent, C., Marcillaud-Pitel, C., Pronost, S., 2020. New EHV-1 variant identified. Veterinary Record 186, 573. https://doi.org/10.1136/vr.m1441 

Thieulent C., Hue E., Sutton G., Fortier C., Dallemagne P., Zientara S., Munier-Lehmann H., Hans A., Paillot R., Vidalain P.-O., Pronost S., 2020. Identification of antiviral compounds against equid herpesvirus-1 using real-time cell assay screening: Efficacy of decitabine and valganciclovir alone or in combination. Antiviral Research 183, 104931. https://doi.org/10.1016/j.antiviral.2020.104931 

Sutton G., Thieulent C., Fortier C., Hue E., Marcillaud-Pitel C., Pléau A., Deslis A., Guitton E., Paillot R., Pronost S., 2020. Identification of a new equid herpesvirus 1 DNA polymerase (ORF30) genotype with the isolation of a C2254/H752 strain in French horses. Viruses 131; 12 (10) : 1160. https://doi.org/10.3390/v12101160 

Thieulent C., Munier-Lehmann E., Fortier C., Suzanne P., Dallemagne P., Zientara S., Hans A., Paillot R., Vidalain P.-O., Pronost S., Hue E., 2020. Screening of potential antiviral molecules against equid herpesvirus-1 using cellular impedance measurement: dataset of 2,891 compounds. Data in Brief 33, 106492. https://doi.org/10.1016/j.dib.2020.106492 

Thieulent C. J., Hue E., Fortier C., Dallemagne P., Zientara S., Munier-Lehmann H., Hans A., Fortier G., Pitel P-H., Vidalain P-O., Pronost S., 2019. Screening and evaluation of antiviral compounds against Equid alphaherpesviruses using an impedance-based cellular assay. Virology 526, 105-116. https://doi.org/10.1016/j.virol.2018.10.013 

PUBLISHED WORKS IN MYBIBLIOGRAPHY

ORCID ID

Grant Funding

Modulation of canine airway epithelial responses by Mycoplasma spp. and their impact on canine respiratory coronavirus co-infection dynamics, Veterinary Translational Science Award (VTSA), LSU Vet Med, 2024 – 2025, $10,000

Tropism, Growth Kinetics and Transcriptional Profiling of Equine Infectious Anemia Virus Infection in Peripheral Blood Mononuclear Cells of Horses and Donkeys, USDA Animal Health and Disease Research Program (Section 1433), 2024 – 2025, $9,615

Rapid detection of latent equine respiratory herpesviruses in peripheral blood mononuclear cells and peripheral tissues by multiplex one-step RT-qPCR assays, EHSP Charles V. Cusimano Research Grant, 2023 – 2024, $12,000