Education

Post-doctoral fellowship, Duke University

NIH-T32 Trainee, Viral Oncology Training Grant, Duke University MedicalCenter

PhD, Louisiana State University, 2016

BSc, University of North Carolina, 2011

Research Interest

My lab focuses on the development of immune-competent small animal models to study gamma herpesviruses (KSHV and EBV) and human papillomavirus (HPV) in the context of viral oncogenesis and immune deficiency. We aim to improve our understanding of how these viruses drive cancer development and immune evasion, particularly in settings of HIV-associated immune suppression.

My research integrates virology, immunology, cancer biology, and molecular genetics, with major areas of focus including:

• Guinea pig models of KSHV infection, characterizing viral latency, immune responses, and potential oncogenic drivers.
• Developing a guinea pig model of EBV infection to study the link between chronic EBV infection, cancer, and autoimmune disease.
• HPV-associated cancers in small animal models, exploring host restriction and immune modulation in guinea pigs.
• Virus-driven oncogenesis in immune-deficient settings, particularly in the context of HIV-associated immune deficiency.
• Our work aims to establish physiologically relevant small animal models that better replicate human immune responses to viral oncogenesis, supporting the development of novel therapeutic and vaccine strategies.

Teaching Interest

I am interested in teaching molecular biology techniques, immunology, virology, and microbiology. 

Clinical Interest

I collaborate with clinical veterinarians and pathologists to investigate virus-associated cancers in animal populations and use patient-derived samples to study viral pathogenesis in immunocompromised hosts.  

Awards & Honors

2021, Faculty Opinions: Associate Faculty Member Travel Grant Critical Care & Emergency Medicine Faculty 

2018, Viral Oncology Training Grant Trainee, Duke University Medical Center Department of Molecular Genetics and Microbiology (Renewal) 

2017, Best Dissertation Award for 2016, Louisiana State University School of Veterinary Medicine 

2017, Viral Oncology Training Grant Trainee, Duke University Medical Center Department of Molecular Genetics and Microbiology 

2016, Hannelore and Johannes Storz student travel award, Louisiana State University 
School of Veterinary Medicine Department of Pathobiological Sciences 

2015, 3rd Place Graduate Student Poster, SE Regional IDeA Conference, Biloxi, Mississippi 

2015, Hannelore and Johannes Storz student travel award, Louisiana State University 
School of Veterinary Medicine Department of Pathobiological Sciences 

2012, Research Fellowship, Louisiana Board of Regents 

Publications

Nabi R, Chouljenko VN, Musarrat F, Davis ME, Mohan H, Ghavimi R, et al. The Novel Oncolytic Herpes Simplex Virus Type‐1 (HSV‐1) Vaccine Strain VC2 Constitutively Expressing GM‐CSF Causes Increased Intratumoral T Cell Infiltration and Inhibition of Tumor Metastasis in the 4T1/Balb/c Mouse Model of Stage Four Breast Cancer. Journal of Medical Virology. 2025;97(2):e70220.

Stanfield BA, Ruiz E, Chouljenko VN, Kousoulas KG. Guinea pig herpes like virus is a gamma herpesvirus. Virus Genes. 2024;60(2):148-58.

Saade DI, Liu CC, Mills EP, Stanfield B, Thieulent CJ, Chouljenko VN, et al. Replication kinetics and cytopathic effect of feline calicivirus in feline corneal epithelial cells. Veterinary Ophthalmology. 2024.

Ramulu NC, Thota S, Stanfield B, Kousoulas K, Francis J. Oncolytic hsv-1 (vc2) gm-csf regulates the proinflammatory profile suggestive of an immunomodulatory effect in PDAC cells. Cancer Research. 2024;84(6_Supplement):1605-.

Hussain MT, Stanfield BA, Bernstein DI. Small animal models to study herpes simplex virus infections. Viruses. 2024;16(7):1037.

Chouljenko AV, Stanfield BA, Melnyk TO, Dutta O, Chouljenko VN. A Repetitive Acipenser gueldenstaedtii Genomic Region Aligning with the Acipenser baerii IGLV Gene Cluster Suggests a Role as a Transcription Termination Element Across Several Sturgeon Species. International Journal of Molecular Sciences. 2024;25(23):12685.

Singha M, Pu L, Srivastava G, Ni X, Stanfield BA, Uche IK, et al. Unlocking the potential of kinase targets in cancer: insights from CancerOmicsNet, an AI-driven approach to drug response prediction in cancer. Cancers. 2023;15(16):4050.

White TM, Stanfield BA, Bonavita CM, Rudd JS, Cardin RD. Development of a mouse salivary gland-derived mesenchymal cell line for immunological studies of murine cytomegalovirus. Plos one. 2022;17(8):e0265479.

White TM, Bonavita CM, Stanfield BA, Farrell HE, Davis-Poynter NJ, Cardin RD. The CMV-encoded G protein-coupled receptors M33 and US28 play pleiotropic roles in immune evasion and alter host T cell responses. Frontiers in Immunology. 2022;13:1047299.

Van Orden K, Santos J, Stanfield B, Frost LS, Ruditsky A, Foster A, et al. Bovine versus porcine acellular dermal matrix for abdominal wall herniorrhaphy or bridging. European Journal of Trauma and Emergency Surgery. 2022:1-9.

Uche IK, Stanfield BA, Rudd JS, Kousoulas KG, Rider PJ. Utility of a Recombinant HSV-1 Vaccine Vector for Personalized Cancer Vaccines. Frontiers in molecular biosciences. 2022;9:832393.

Uche IK, Stanfield B, Rudd J, Kousoulas K, Rider PJ. Prospects for personalized cancer vaccines: The oncolytic and immunotherapeutic HSV-1 (VC2) virus expressing an ovalbumin (OVA) antigen elicits robust anti-OVA immune responses. Cancer Research. 2022;82(12_Supplement):3570-.

Stanfield BA, Bravo FJ, Dixon DA, Chouljenko VN, Kousoulas KG, Bernstein DI. Cross protective efficacy of the Non-Neurotropic live attenuated herpes simplex virus type 1 vaccine VC-2 is enhanced by intradermal vaccination and deletion of glycoprotein G. Vaccine. 2022;40(42):6093-9.

Singha M, Pu L, Stanfield BA, Uche IK, Rider PJ, Kousoulas KG, et al. Artificial intelligence to guide precision anticancer therapy with multitargeted kinase inhibitors. BMC cancer. 2022;22(1):1211.

Stanfield BA, Soderblom EJ, Palmer S, Sullenger B, Purves T, Welty-Wolf K, et al. Histone Deacetylase 3 Associates With Key Regulators of NFκB Signaling in Lung Epithelial Cells Under Stimulation With LPS. 2021.

Stanfield BA, Purves T, Palmer S, Sullenger B, Welty-Wolf K, Haines K, et al. IL-10 and class 1 histone deacetylases act synergistically and independently on the secretion of proinflammatory mediators in alveolar macrophages. PLoS One. 2021;16(1):e0245169.

Stanfield BA, Kousoulas KG, Fernandez A, Gershburg E. Rational Design of Live-Attenuated Vaccines against Herpes Simplex Viruses. Viruses. 2021;13(8):1637.

Panthi S, Schmitt PT, Lorenz FJ, Stanfield BA, Schmitt AP. Paramyxovirus-like particles as protein delivery vehicles. Journal of virology. 2021;95(20):10.1128/jvi. 01030-21.

Kasotakis G, Stanfield B, Haines K, Vatsaas C, Alger A, Vaslef SN, et al. Acute Respiratory Distress Syndrome (ARDS) after trauma: improving incidence, but increasing mortality. Journal of critical care. 2021;64:213-8.

Kasotakis G, Chen W, Corcoran DL, Howrylak JA, Englert J, Sullenger BA, et al. Differential Transcriptomic Profiling Between Patients With Sepsis-Induced Ards And Sepsis Without Ards. 2021.

Bonavita CM, White TM, Stanfield BA, Cardin RD. Characterization of murine cytomegalovirus infection and induction of calcification in Murine Aortic Vascular Smooth Muscle Cells (MOVAS). Journal of virological methods. 2021;297:114270.

Stanfield B, Palmer S, Purves T, Haines K, Agarwal S, Kasotakis G. 565: IL-10 SUPPRESSES IL-12B SYNTHESIS THROUGH HISTONE DEACETYLASE 3 MEDIATION IN ALVEOLAR MACROPHAGES. Critical Care Medicine. 2020;48(1):263.

Stanfield B, Fernandez-Moure J, Haines K, Rowell SE, Agarwal S, Kasotakis G. Histone Deacetylase 3 Associates with Key Regulators of NFkB Signaling in Human Lung Epithelial Cells under Stimulation with LPS. Journal of the American College of Surgeons. 2020;231(4):e59.

Naidu SK, Nabi R, Cheemarla NR, Stanfield BA, Rider PJ, Jambunathan N, et al. Intramuscular vaccination of mice with the human herpes simplex virus type-1 (HSV-1) VC2 vaccine, but not its parental strain HSV-1 (F) confers full protection against lethal ocular HSV-1 (McKrae) pathogenesis. PLoS One. 2020;15(2):e0228252.

Manjappachar N, Cuenca J, Heatter J, Martin P, Sterling B, Nates J, et al. 566: CHECKPOINT INHIBITORS AND POTENTIALLY FATAL IMMUNE-RELATED ADVERSE EVENTS: A CASE SERIES. Critical Care Medicine. 2020;48(1):264.

Stanfield BA, Rider PJ, Caskey J, Del Piero F, Kousoulas KG. Intramuscular vaccination of guinea pigs with the live-attenuated human herpes simplex vaccine VC2 stimulates a transcriptional profile of vaginal Th17 and regulatory Tr1 responses. Vaccine. 2018;36(20):2842-9.

Stanfield BA, Pahar B, Chouljenko VN, Veazey R, Kousoulas KG. Vaccination of rhesus macaques with the live-attenuated HSV-1 vaccine VC2 stimulates the proliferation of mucosal T cells and germinal center responses resulting in sustained production of highly neutralizing antibodies. Vaccine. 2017;35(4):536-43.

Stanfield BA, Luftig MA. Recent advances in understanding Epstein-Barr virus. F1000Research. 2017;6.

Liu SA, Stanfield BA, Chouljenko VN, Naidu S, Langohr I, Del Piero F, et al. Intramuscular immunization of mice with the live-attenuated herpes simplex virus 1 vaccine strain VC2 expressing equine herpesvirus 1 (EHV-1) glycoprotein D generates anti-EHV-1 immune responses in mice. Journal of virology. 2017;91(12):10.1128/jvi. 02445-16.

Liu SA, Haque M, Stanfield B, Andrews FM, Roy AA, Kousoulas KG. A recombinant fusion protein consisting of West Nile virus envelope domain III fused in-frame with equine CD40 ligand induces antiviral immune responses in horses. Veterinary microbiology. 2017;198:51-8.

Stanfield BAD. Immunopathogenesis Of Herpes Simpex Viruses: Design And Testing Of Novel Vaccines To Prevent Herpes Viral Infections. 2016.

Haque M, Stanfield B, Kousoulas KG. Bovine herpesvirus type-1 glycoprotein K (gK) interacts with UL20 and is required for infectious virus production. Virology. 2016;499:156-64.

Stanfield B, Kousoulas KG. Herpes simplex vaccines: prospects of live-attenuated HSV vaccines to combat genital and ocular infections. Current clinical microbiology reports. 2015;2:125-36.

Stanfield BA, Stahl J, Chouljenko VN, Subramanian R, Charles A-S, Saied AA, et al. A single intramuscular vaccination of mice with the HSV-1 VC2 virus with mutations in the glycoprotein K and the membrane protein UL20 confers full protection against lethal intravaginal challenge with virulent HSV-1 and HSV-2 strains. PloS one. 2014;9(10):e109890.

Iyer AV, Pahar B, Chouljenko VN, Walker JD, Stanfield B, Kousoulas KG. Single dose of glycoprotein K (gK)-deleted HSV-1 live-attenuated virus protects mice against lethal vaginal challenge with HSV-1 and HSV-2 and induces lasting T cell memory immune responses. Virology journal. 2013;10:1-11.

Courchesne MJ, White MC, Stanfield BA, Frampton Jr AR. Equine herpesvirus type 1-mediated oncolysis of human glioblastoma multiforme cells. Journal of virology. 2012;86(5):2882-6.

MYNCBI BIBLIOGRAPHY

Grant Funding

Current: Pilot Project – Center for Pre-Clinical Cancer Research, Developing Guinea Pig Models for KSHV Infection

Pending: NIH R21 – Developing Guinea Pig Models for KSHV Infection

Pending: NIH Contract – Mouse and Guinea Pig Models of Cytomegalovirus and Herpes Simplex Virus Infections.

Pending: NIH R01 – Guinea Pig Models to Study HIV-Associated Kaposi’s Sarcoma

Pending: NIH R01 – Guinea Pig Models to Study Human Epstein Barr Virus